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KMID : 0363320140350010079
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Journal of Korean Oriental Internal Medicine
2014 Volume.35 No. 1 p.79 ~ p.91
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Anti-cancer Effects of Palbohoichoon-tang on Neuroblastoma Cells
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An Jung-Hwan
Cho Mun-Young
Woo Chan
Shin Yong-Jeen
Shin Sun-Ho
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Abstract
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Objectives : To investigate the anti-cancer effect of Palbohoichoon-tang (PBHCT) extracts.
Methods : The cell viability was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MMT) assay and cell morphological changes were microscopically analyzed after staining with 10 μM 2-[4-amidinophenyl]-6-indolecarbamidine dihydrochloride (DAPI) and TUNEL. We also analyzed expression of Bcl2, BclxL, Bax, procaspase-3, procaspase-9, and procyclic acidic repetitive protein (PARP) by western blot method.
Results : Observations showed that PBHCT induced the apoptotic cell death proved by increased sub-G1 phase cell
population, apoptotic body formation and chromatin condensation. Western blot analysis of total cell lysates revealed that the PBHCT induced cleavage of caspase-9, caspase-3 and poly (ADP-ribose) polymerase (PARP). In addition, PBHCT dose-dependently increased the activity of caspase-9, caspase-3 and PARP-1. Furthermore, PBHCT reduced anti-apoptotic Bcl2, BclxL expression which contributed to the loss of mitochondrial membrane potential and the activations of caspase-9 and caspase-3.
Conclusions : These findings suggest that PBHCT exerts anti-cancer effects on human neuroblastoma SH-SY5Y cells by
inducing apoptotic death via down-regulation of anti-apoptotic proteins such as Bcl2 and BclxL, up-regulation of pro-apoptotic proteins such as Bax, and activation of caspase cascades and PARP-1.
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KEYWORD
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SH-SY5Y cell, Anti-cancer effect, Palbohoichoon-tang (PBHCT)
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